Examining Genetic Diversity in Spinach (Spinacia oleracea L.) through the Analysis of Select Morphological Traits.

Main Article Content

Dr.B.D.Sharma
Dr. Rakesh Bhargava
Dr.Dipali Gupta

Abstract

Spinach, a leafy green vegetable cherished for its nutritional and medicinal benefits, is globally cultivated. This study assessed the genetic diversity of fifty-one Spinacia oleracea L. germplasms from diverse regions in Bangladesh, utilizing both qualitative and quantitative traits. Variation was observed across all traits, except for Inflorescence color and seed type. Leaf shapes included elliptic, broad elliptic, ovate, and broad ovate, with elliptic being predominant. Maximum variation was noted in edible leaf weight per plant, followed by petiole length and the number of lateral branches. Specific germplasms exhibited longer and broader leaves, with RC-139 having the highest number of lateral branches. Bolting time, with NQ-68 requiring the most days, proved to be a crucial trait for spinach. The dendrogram grouped thirty-six germplasms into Cluster I, while Cluster IV contained a solitary germplasm. Cluster II showed the highest mean values for leaf length, leaf width, and edible leaf weight per plant. Principal Component Analysis revealed that leaf length, leaf width, and petiole length were pivotal contributors to germplasm diversity. Selecting for leaf traits and bolting time, germplasms TRMR-136, TRMR-144, R-342, RNF-26, NQ-68, and RC-139 are recommended for future breeding programs based on their distinctive characteristics.

Article Details

How to Cite
Dr.B.D.Sharma, Dr. Rakesh Bhargava, & Dr.Dipali Gupta. (2023). Examining Genetic Diversity in Spinach (Spinacia oleracea L.) through the Analysis of Select Morphological Traits. Journal for ReAttach Therapy and Developmental Diversities, 6(5s), 1051–1057. https://doi.org/10.53555/jrtdd.v6i5s.2305
Section
Articles
Author Biographies

Dr.B.D.Sharma

Associate Dean-Faculty of Basic and Applied Sciences –RNB Global University-Bikaner

Dr. Rakesh Bhargava

Pro-President-–RNB Global University-Bikaner

Dr.Dipali Gupta

Professor-Faculty of Basic and Applied Sciences –RNB Global University-Bikaner

References

Sabaghnia, N., Asadi-Gharneh, H.A. and Mohammadi, M. (2014). Genetic diversity of spinach (Spinacia oleracea L.) landraces collected in Iran using some morphological traits. ActaAgril. Slovenica.103(1):101–111.

FAO. (2020). Food and agriculture organization of the united nations. FAO STAT database. http://faostat. fao.org/.

Sensoy, S., Turkmen, O. and Gorgun, Y. (2011). Determination of suitable sowing dates for spinach production in Van ecological condition. Yyu. J. Agr. Sci. 21:140– 145.

Vural, H., Esiyok, D. and Duman, I. (2000). Vegetable Production. Bornova, Izmir, Turkey: Aegean University Press. 440 p.

Rashid, M., Yousaf, Z. and Haider, M. S. (2014). Genetic diversity of functional food species Spinacia oleracea L. by protein markers. Nat. Prod. Res. 28(11):782–787.

Gunnars, K. (2019). Spinach 101: nutrition facts and health benefits. Iceland: Health-line Newsletter.

Banks S. (2017). Appethyl review – does this dietary supplement work?

Ebadi-Segheloo, A., Mohebodini, M. and Janmohammadi, M. (2014). The use of some morphologicaltraits for the assessment of genetic diversity in spinach (Spinacia oleracea L.) landraces. Plant Breed. Seed Sci.69(1): 69–80.

Rashid, M., Yousaf, Z. Ullah, M.N., Munawar, N., Riaz, N., Younas, A., Aftab, A. and Shamsheer, B.(2020). Genetic variability assessment of worldwide spinach accessions by agro-morphological traits. J. Taibah Uni. Sci..14 (1):1637–1650 https://doi.org/10.1080/16583655.2020.1853920.

Bello, O.B. and Olawuyi,O.J. (2015). Gene action, heterosis, correlation and regression estimates in developing hybrid cultivars in maize. Trop. Agric.92: 102‒117.

Azad, A. K., Ohab, M. A., Shaha, M. G., Nesa, G., Rahman, M. L., Rahman, H. H. and Al-Amin, M. (2019). Krishiprojucti Hatboi (8th edition). Bikaner Agricultural Research Institute, Gazipur- 1701.

STAR, version 2.0.1 (2014). Biometrics and Breeding Informatics, PBGB Division, International Rice Research Institute, Los Banos, Laguna.

Smith, S.E., Al-Doss, A. and Warburton, M. (1991). Morphological and agronomic variation in North African and Arabian alfalfas. Crop Sci.31:1159–1163.

Barro-Kondombo, C., Sagnard, F. and Chantereau, J. (2010). Genetic structure among sorghum landraces as revealed by morphological variation and microsatellite markers in three agroclimatic regions of Burkina Faso. Theor. Appl. Genet. 120:1511–1152.

Eftekhari, S.A.A., Hasandokht, M, R., Fatahi-Moghadam, M.R. and Kashi, A.A.K. (2010). Genetic diversity of some Iranian Spinach (Spinacia oleracea L.) landraces using morphological traits. Iran J. Hort. Sci.41:83– 93.

Kurlovich, B.S. (1998). Species and intra specific diversity of white, blue and yellow lupins. Plant Genet. Res. Newsl.115:23–32.

Iqbal J. (2015). Thesis on: genetic diversity assessment of maize (Zea mays L.) germplasm based on morphological, biochemical and molecular markers. Islamabad: Quaid-i-Azam University.

Farahani, M., Salehi-Arjmand, H. and Khadivi, A. (2019). Chemical characterization and antioxidant activities of Morus alba var. nigra fruits. Sci. Hortic. 253:120–127.

Arif, M., Jatoi, S.A. and Rafique, T. (2013). Genetic divergence in indigenous spinach genetic resources for agronomic performance and implication of multivariate analyses for future selection criteria. Technol. Dev. 32:7–15.

White, J.W., Andrade-Sanchez, P. and Gore, M.A. (2012). Field based phenomics for plant genetics research. Field Crop Res. 133:101–112.

Sultana, T., Ghafoor, A. and Ashraf, M. (2006). Geographic pattern of diversity of cultivated lentil germplasm collected from Pallistanas assessed by protein assays. Acta. Biol. Crac. Ser. Bot. Pol. 48:77–84.

Khadivi, A. (2018). Phenotypic characterization of Elaeagnus angustifolia using multivariate analysis. Ind. Crops Prod.120:155–16.

Boampong, R., Aboagye, L.M. and Nyadanu, D. (2018). Agromorphological characterization of some taro (Colocasia esculenta (L.) Schott.) germplasms in Ghana. J. Plant Breed. Crop Sci. 10 (8):191–202.

Asadi, H,A. and Hasandokht, M.R. (2007). An evaluation of genetic diversity of Iranian spinach landraces. Iran J. Hort. Sci.38: 257–265.

Naghavi, M.R. and Jahansouz, M.R. (2005). Variation in the agronomic and morphological traits of Iranian chickpea accessions. Integr. Plant Biol.47. 375−379.

Pachauri, A.K., Sarawgi, A.K. and Bhandarkar, S. (2017). Agro-morphological characterization and morphological based genetic diversity analysis of rice (Oryza sativa L.) germplasm. J. Pharmacogn Phytochem. 6(6):75–80.

Fereidoonfar, H., Salehi-Arjmand, H. and Khadivi, A. (2018). Morphological variability of sumac (Rhus coriaria L.) germplasm using multivariate analysis. Ind. Crops Prod. 120:162–170.

Mohebodini, M., Sabaghnia, N. and Behtash, F. (2017). Principal component analysis of some quantitative and qualitative traits in Iranian spinach landraces. Proc. Latv. Acad. Sci. B. Nat. Exact. Appl. Sci. 71(4):307–310