Study On Green Synthesis And Characterization Of Copper, Manganese Nanoparticles In T.Populnea For Biomedical Applications
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Abstract
Nanotechnology is a broad interdisciplinary field with many applications in science & technology. These Nanoparticles are 1-100nm in dimension. Locally available plant namely Thespesia populnea commonly called Portia tree leaves and figs were taken for nanoparticle isolation. The solvent most widely used are water, Copper sulphate & Manganese oxide mixture at PH 5 -7, temperature between 50-700 C and Concentrations 1:2, 1:3 respectively. The mixtures after incubation centrifuged and the pellet was dried in oven at 70℃ for 2hours and the formed copper and Manganese nanoparticles were isolated. Totally 32 samples of copper & Manganese nanoparticles with different parameters have been collected. 0.1g of each sample is taken and 1ml of DMSO (Dimethyl Sulfoxide) to each tube and mixed them thoroughly. Then 10µl of the prepared solution was added and the sample is subjected to UV-Visible spectrophotometer at 200nm, 220nm, 240nm, 250nm, 300nm, 350nm and 400nm, SEM at 500 – 94,300X, XRD at 2ɵ = 100– 900 for characterization. Antibacterial and antifungal activity performed i.e sterilized and solidified nutrient agar and PDA loaded with 100µl of Gram positive bacteria, Gram negative bacteria, fungal organisms through disc diffusion method. Cu NP’s acts against upon Gram positive Bacteria Clostridium Perfringens, Gram negative Bateria Aeromonas hydrophila, Salmonella enterica & Fungi like Phytophthora infestans and Mn NP’s acts against upon Gram positive bacteria Bacillus subtilis , Gram negative bateria Salmonella enterica & Fungi like Candida albicans
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