Isolation And Identification of Flavonoids from Madhuca Indica (Sapotaceae): A Comprehensive Phytochemical Analysis

Main Article Content

Mudita Mishra
Seema Tomar
Ashutosh Mishra

Abstract

Madhuca indica is a forest tree that grows in Malaysia and central and northern India. It is frequently seen in Bangladesh and other regions of the Indian subcontinent. These parts are used to treat a wide range of illnesses, including infestations of the helm, low testosterone count, headaches and migraines bloating, infections, rheumatic arthritis, cholera, paralysis, snakebite, debility, tonsillitis, influenza, piles, arthritic pain, and bacterial infections. They are also used as a purifying blood and an antidote to a poisonous substance


In current flavonoids contained in Madhuca indica aerial parts were extracted, identified and characterized. Sequential Soxhlet extraction was subjected to preliminary phytochemical screening and flavonoid quantification. The results showed that maximum yield of the flavonoid content (% w/w) were obtained from ethyl acetate extract. This ethyl acetate extract was subjected to column chromatography for isolation of flavonoid. Characterization of isolated flavonoid was done by UV, IR, 1H NMR and MS. On the basis of chemical and spectral analysis structure were elucidated as quercetin, rutin apigenin and naringenin flavonoid.

Article Details

How to Cite
Mudita Mishra, Seema Tomar, & Ashutosh Mishra. (2023). Isolation And Identification of Flavonoids from Madhuca Indica (Sapotaceae): A Comprehensive Phytochemical Analysis. Journal for ReAttach Therapy and Developmental Diversities, 6(1), 1539–1549. https://doi.org/10.53555/jrtdd.v6i1.2732
Section
Articles
Author Biographies

Mudita Mishra

Faculty of Pharmaceutical Sciences, Motherhood University, Roorkee

Seema Tomar

Faculty of Pharmaceutical Sciences, Motherhood University, Roorkee

Ashutosh Mishra

Department of Pharmaceutical Science, Acharya Narendra Deo College of Pharmacy Babhnan Gonda, Uttar Pradesh

References

Figueira I, Menezes R, Macedo D, Costa I, Nunes dos Santos C. Polyphenols beyond barriers: a glimpse into the brain. Current neuropharmacology. 2017 May 1;15(4):562-94.

Nijveldt RJ, Van Nood EL, Van Hoorn DE, Boelens PG, Van Norren K, Van Leeuwen PA. Flavonoids: a review of probable mechanisms of action and potential applications. The American journal of clinical nutrition. 2001 Oct 1;74(4):418-25.

Miean KH, Mohamed S. Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants. Journal of agricultural and food chemistry. 2001 Jun 18;49(6):3106-12.

Karak P. Biological activities of flavonoids: An overview. Int. J. Pharm. Sci. Res. 2019 Apr;10(4):1567-74.

Quamar MF, Kar R, Thakur B. Vegetation response to the Indian Summer Monsoon (ISM) variability during the Late-Holocene from the central Indian core monsoon zone. The Holocene. 2021 Jul;31(7):1197-211.

Reddy IS. Madhuca indica: An untapped forest tree for its medicinal uses. Pharma Innov J. 2022;11(3):1747-51.

Lakshmiprasanna, K. and Aparna, K. Madhuca indica - A Tree for All Reasons. Biotica Research Today. 2, 2020, 469-471.

Khan S, Zahan D, Das R, Nasrin D, Ahsan S, Ahmed R, Sadat AF, Bashar AB, Nahar N, Rahmatullah M. Antihyperglycemic activity studies with methanol extract of Madhuca indica JF Gmel. leaves and Paederia foetida L. stems in mice. Adv Nat Appl Sci. 2011 Apr 1;5(2):122-6.

Sengar NP, Singh AR. A Text Book of Pharmacognosy. Pharmamed press, Hyderabad, Edition. 2009;1:44-5.

Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas. Springer Science & Business Media; 1996.359-364.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia, 1 (1), 98-106.

Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis. 2002 Jul 1;10(3).