Enhancing Antibacterial Activity Of Methanol Extract Of Phoenix Dactylifera Leaves Against Salmonella Entrica And Escherichia Coli Isolates
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Abstract
The present research detects the effectiveness and estimation of Methanol Extract of phoenix dactylifera (leaves palm extract) as antibacterial activity against selected Gram negative bacteria (Salmonella entrica and
Escherichia coli ) , which may be developed into new-fangled, harmless, and more efficient agents microbial infections. was determined by Serial doubling dilution of the plant extract in sterile nutrient broth to determine the minimum inhibitory concentration and achieved six different concentrations of 200µg/ml, 100µg/ml, 50µg/ml, 25 µg/ml, and 12.5µg/ml and 6.25 µg/ml, Its appeared as negative result (not have any zone of inhibition) in deferent concentration used.
When used the synergistic effect of Methanol Extract of dactylifera leaves against Salmonella entrica with different antibiotic showed Ciprofloxacin (CIP), Trimethoprim (TMP) ,resistance in deferent concentration but Cefataxime ( CTX) sensitive in all concentration except ( 12.5 µg/ml, 6.250 µg/ml) were resistance .While Amikacin (AK) sensitive in concentration (50 µg/ml, 25 µg/ml) and Doxycycline (DO) sensitive in concentration (50 µg/ml) only .
Whereas the synergistic effect of dactylifera leaves against Escherichia coli were resisting in all antibiotics used except Cefataxime (CTX) sensitive in all concentration that used in the research and Amikacin (AK) sensitive in concentration (12.5 µg/ml, 6.250 µg/ml).
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References
Whitman, T.J. (2008). Community associated methicillin - resistant Staphylococcus aureus skin and soft tissue infections. Dis. Month, 54: 780-786.
Christopher R. Frei, Brittany R. Makos , Kelly R. Daniels, Christine U. Oramasion (2010). Emergence of community-acquired methicillin-resistant Staphylococcus aureus skin and soft tissue infections as a common cause of hospitalization in United States children. Pediatr Surg, 45(10):1967-74. DOI: 10.1016/j.jpedsurg.2010.05.009
M. Iftikhar Hussain, Mohammad H. Semreen, Abdallah hanableh, Muhammad Nasir Khan Khattak, Ismail Saadoun, Islam M. Ahmady, Muath Mousa, Nora Darwish, Wameed Radeef, and Sameh S. M. Soliman, (2019) . Phenolic Composition and Antimicrobial Activity of Different Emirati Date (Phoenix dactylifera L.) Pits: A Comparative Study. Plants (Basel). ; 8(11): 497. Doi: 10.3390/plants8110497
Qadoos, H. A., Dhafari, H. S., Al Marzooqi, D. A., Kumarappan, A., & Nazir, A. , (2017) . Phenolic content and antimicrobial activities of date palm (Phoenix dactylifera L.) fruits and leaves. Food Biology, 11-15.
John, J. A., & Shahidi, F. (2019). Phenolic content, antioxidant and anti-inflammatory activities of seeds and leaves of date palm (Phoenix dactylifera L.). Journal of Food Bioactives, 5, 120-130.
Sahyon, H. A., & Al-Harbi, S. A. (2020). Antimicrobial, anticancer and antioxidant activities of nano-heart of Phoenix dactylifera tree extract loaded chitosan nanoparticles: In vitro and in vivo study. International Journal of Biological Macromolecules.
Al-Shahib, W.; Marshall, R.J. (2003). Fatty acid content of the seeds from 14 varieties of date palm Phoenix dactylifera L. Int. J. Food Sci. Technol., 38: 709-12.
M. Iftikhar Hussain; Mohammad Semreen; Abdallah Shanableh ; Sameh Soliman (2012). Phenolic Composition and Antimicrobial Activity of Different Emirati Date (Phoenix dactylifera L.) Pits: A Comparative Study. Journal of Medicinal Plant Research 6(2).
DOI:10.5897/JMPR11.1380
Kahkashan Perveen (2012). Antibacterial activity of Phoenix dactylifera L. leaf and pit extracts against selected Gram negative and Gram positive pathogenic bacteria. Journal of Medicinal Plant Research 6(2). DOI:10.5897/JMPR11.1380 .10- MacFaddin (2000). Biochemical Tests for Identification of Medical Bacteria. Lippincott Williams & Wilkins.
Clinical and Laboratory Standards Institute (CLSI), 2018. Performance standards for antimicrobial susceptibility testing (28th ed.), CLSI supplement M100, Wayne, PA.
Tauqeer Hussain Mallhi ; M. Imran Qadir; Mazyoud Ali; Atta Ur Rehman , (2014). Ajwa Date (Phoenix dactylifera): An Emerging Plant in Pharmacological Research . PAK J PHARM SCI 27(3):607-16
Shatha Mousa mlaghee Al-safi; Kifah fadhil hasson Al-Shabaa ; Saadia S. Mehdy AL-Zeiny ;Aiman Mohammed Baqir Al-Dhalimy ,(2020). Synergistic and antimicrobial effects of leaves oil(phoenix dactylifera) agonist gram negative bacteria. Sys Rev Pharm 2020;11(11):177-181 .
Wikler M.A. ,(2019). Methods for Dilution Antimicrobial Susceptibility Test for Bacteria that Grow Aerobically; Approved Standard M7-A8. Available. Online: https://simpleshowoflove.weebly.com/uploads/1/4/0/7/14073276/agar_dilution_assay.
Ali, H., Qaiser, M. (2009). The ethnobotany of Chitral valley, Pakistan with particular reference to medicinal plants. Pak. J. Bot., 41: 2041.
Al-zeiny, S. S. M. (2018). Determination of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MAC) of seed methanolic extract of Peganumharmala L. against Gram negative bacteria. Kufa Journal For Veterinary Medical Sciences, 9(1), 26-34
Doi, Y., Park, Y. S., Rivera, J. I., Al-Tamimi, M., Abu-Raideh, J., Albalawi, H., ... & Saleh, S. (2020). Effective oral combination treatment for extended-spectrum beta-lactamase-producing Escherichia coli. Clinical Infectious Diseases, 70(12), iii-iv.
Kuang, D., Zhang, J., Xu, X., Shi, W., Chen, S., Yang, X., ... & Meng, J. (2018). Emerging high-level ciprofloxacin resistance and molecular basis of resistance in Salmonella enterica from humans, food and animals. International journal of food microbiology, 280, 1-9.
Al-Daihan, S., & Bhat, R. S. (2012). Antibacterial activities of extracts of leaf, fruit, seed and bark of Phoenix dactylifera. African Journal of Biotechnology, 11(42), 10021-10025
Qadrie, Z.L., Jacob, B., Anandan, R., Rajkapoor, B. and Ulla, M.R. (2009). Antibacterial activity of ethanolic extract of Indoneesiella Echioides (l) Nees evaluated by the filter paper disc method. Pak. J. Pharm. Sci., 22: 123-5.
Nizar, M.A., El Sukhon, S., Msameh, Y., and Abdul-Karim, J.S. (1999). Effect of date fruits, Phoenix Dactyliferia L., on the hemolytic activity of Streptolysin O. Pharma. Biol., 37: 335-9.
Puupponen, P.R., Nohynek, L., Meiier, C., Kahkonen, M., Heinonen, M., Hopia, A., and Caldentey KM (2001). Antimicrobial properties of phenolic compounds from berries. J. Appl. Microbiol., 90: 494-507.
Kahkashan, P., Najat, A. B. and Dina, A. W. (2012). Antibacterial activity of Phoenix dactylifera L. leaf and pit extracts against selected Gram negative and Gram positive pathogenic bacteria. Journal of Medicinal Plants Research . 6(2) : 296-300.
Kahkashan Perveen; Najat A. Bokahri , 2020 . Comparative analysis of chemical, mineral and in-vitro antibacterial activity of different varieties of date fruits from Saudi Arabia. Saudi J Biol Sci. 27(7): 1886– 91.
DOI: 10.1016/j.sjbs.2019.11.029
Cutter, C.V. (2000). Antimicrobial effect of herb extracts against Escherichia coli, Listeria monocytogenes and Salmonella typhimurium associated with beef. J. Food Prot., 63: 601-7.
Khademalhosseini A. A.; Tabatabaei A.; Akbari P.; Fereidouni M. S. And Akhlaghi. M. (2015) .Comparison of in vivo antiseptic and in vitro antimicrobial effects Extract of Phoenix dactylifera methanolic extract with Betadine. Journal of Coastal Life Medicine; 3(1): 70-7.