"Photophysics Of Fluorescent Nanoparticles Concerning, Organic Dyes And Design Principles"

Abstract

Fluorescent nanoparticles (Nanoparticles) embedded with molecular organic fluorophores show promise in bioanalysis and imaging due to their high brightness and photostability. However, close packing of these dyes within nanoparticles often leads to fluorescence quenching and altered spectral properties. This perspective discusses the origins of these issues, various nanoparticle designs, and strategies to mitigate dye–dye interactions and quenching. The aim is to provide a framework to understand the supramolecular mechanisms affecting the photophysics of dye-based Nanoparticles, aiding in the design of improved fluorescent nanoparticles.