In Vivo And In Vitro Study on Antioxidant Activity Of Methanolic Extract Of Cryptostegia Grandiflora Leaves In Experimental Animals

Abstract

This study evaluates the antioxidant and anticancer activities of the methanolic extract of Cryptostegia grandiflora leaves (CGME) using both in vitro and in vivo models. The antioxidant potential was assessed through DPPH radical scavenging in vitro and through various enzymatic assays in an animal model. Instrumental analysis such as Column Chromatography, UV, HPTLC, GCMS, and 1H NMR were utilized for compound identification. The results showed that CGME exhibited significant antioxidant activity in the DPPH assay and increased the levels of antioxidant enzymes, including SOD, CAT, GRD, GPX, and GST, while reducing LPO levels in vivo. These findings suggest that CGME has potential as a natural antioxidant with anticancer properties. Further studies are warranted to explore its mechanisms of action and therapeutic potential.