Herbal Mediated Magnesium Oxide Nanoparticles For Root Canal Treatment

Abstract

Oral cavity inhabits wide range of microorganisms which comprises of bacteria, viruses and protozoans.Endodontic disease is a biofilm facilitated infection, and objective in its treatment is the removal of these biofilms from the endodontic canals. Biofilms are aggregates of microorganisms in which cells are frequently embedded in a self-produced matrix of extracellular polymeric substances (EPS) that are adherent to each other and/or a surface.These Nanoparticles can be employed in any past of RCT treatment as in sealers, obturating materials, irrigation, and intracanal medicament. Magnesium oxide Nanoparticles have antibacterial and anti biofilm activities against two cariogenic microorganisms including Streptococcus mutans and Streptococcus sobrinus where S. mutans significantly contributes to dental caries. Also from other inorganic metal oxides magnesium oxide Nanoparticles are nontoxic and relatively easy to obtain.

Materials and method: The study involved invitro setup. The natural teeth extracted due various reasons mostly caries involving the pulp were selected for the study.The anterior teeth were selected and were scaled to remove debris and calculus. The RCT was performed. Dried Teak leaf (5mg)+50ml of ethanol was boiled and filtered. 5ml Magnesium oxide was added to 20ml of distilled water, colour changes.Solution was later kept for drying at 80’C using hot air oven. Later % of lysis analysis was done with blood buffer along with SEM and FTIR analysis.

Results: control of blood with buffer showed a % of lysis of 5 whereas our samples showed around 4.2 which is below 5. It also shows the haemocompatibility in our samples.

Conclusion: Herbal mediated MgONPs using teak extract is showing positive results as antimicrobial activity is shown but it’s activity is less as compared to the control.