Screening Of Amylase Producing Halophilic Bacteria And It’s Phenotypic Identification
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Abstract
Halophilic bacteria are valuable sources of stable extremozymes, particularly for industrial applications requiring high salinity. This study focuses on the screening of amylase-producing halophilic bacteria and their phenotypic identification. Bacterial isolates were cultured, isolated, and morphologically assessed. Microscopic evaluation revealed Gram-negative, motile bacilli. A comprehensive biochemical profile was established, showing positive results for Methyl Red, Voges Proskauer, Citrate, Urease, and Catalase, while testing negative for Indole and Oxidase. Extracellular enzyme screening demonstrated that while the isolates completely lacked protease activity, they exhibited notable cellulase activity, with specific strains (CCMPL003 and CCMPL004) showing high efficacy. These findings suggest that the isolated halophilic strains possess a robust multi-enzymatic profile suitable for biotechnological applications.
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References
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