In-silico identification of microRNA-1224-5p from the Oral squamous cell carcinoma (OSCC) human genome sequence
Main Article Content
Abstract
Background: Oral squamous cell carcinoma (OSCC) is the most common histotype of head and neck cancer, accounting for 90% of all diagnosed cases. The goal of modern cancer research is to develop less invasive techniques that can provide a more complete picture of the cancer profile and make it simple to track the disease's development and therapeutic response. Interestingly, microRNAs (miRNAs or miRs) are studied for their potential role as biomarkers and therapeutic targets in various diseases, including OSCC.
Methodology: In this study, we have used bioinformatic approaches to identify hsa-miR-1224-5p for OSCC using NCBI database, miRbase and target scan. Finally, RNA fold was used to create the secondary structure of hsa-miR-1224-5p.
Results and discussion: Careful evaluation of the secondary structure result showed that hsa-miR-1224-5p has a minimum free energy of – 44.10 kcal. The correlation between hsa-miR-1224-5p and OSCC genome sequence was identified.
Conclusion: These computational approaches have concluded that hsa-miR-1224-5p can be used as a diagnosis, prognosis and effective therapeutic target for treating OSCC. Thus, further research could enlighten the role of hsa-miR-1224-5p in OSCC.
Article Details
References
Badve S, Kumar GL. Predictive Biomarkers in Oncology: Applications in Precision Medicine. Springer; 2018. 642 p.
Taherkhani A, Dehto SS, Jamshidi S, Shojaei S. Pathogenesis and prognosis of primary oral squamous cell carcinoma based on microRNAs target genes: a systems biology approach [Internet]. Vol. 20, Genomics & Informatics. 2022. p. e27. Available from: http://dx.doi.org/10.5808/gi.22038
Palaia G, Pippi R, Rocchetti F, Caputo M, Macali F, Mohsen A, et al. Liquid biopsy in the assessment of microRNAs in oral squamous cell carcinoma: A systematic review. J Clin Exp Dent. 2022 Oct;14(10):e875–84.
Selvakumar SC, K Auxzilia P, Dinesh Y, Senthilmurugan M, Sekar D. MicroRNA-138 and its targets: A therapeutic molecule for oral squamous cell carcinoma. Oral Oncol. 2022 Jul;130:105925.
Sekar D, Murthykumar K, Ganapathy D. miR-206 and its mimics: A predictive biomarker and therapeutic molecule in the treatment of oral cancer. Oral Oncol. 2022 May;128:105849.
Saravanan S, Islam VIH, Thirugnanasambantham K, Sekar D. In Silico Identification of Human miR 3654 and its Targets Revealed its Involvement in Prostate Cancer Progression. Microrna. 2016;5(2):140–5.
Yuan X, Jing Y, Guang M, Zhu J, Wang J, Wang Y, et al. GAS5 alleviates cisplatin drug resistance in oral squamous cell carcinoma by sponging miR-196a. J Int Med Res. 2022 Oct;50(10):3000605221132456.
Sun J. Inflammation, Infection, and Microbiome in Cancers: Evidence, Mechanisms, and Implications. Springer Nature; 2021. 509 p.
Baharvand H, Aghdami N. Advances in Stem Cell Research. Springer Science & Business Media; 2012. 398 p.
Mousa SA. Angiogenesis Inhibitors and Stimulators: Potential Therapeutic Implications. CRC Press; 2000. 162 p.
Jiang Z, Hu H, Hu W, Hou Z, Liu W, Yu Z, et al. Circ-RNF121 regulates tumor progression and glucose metabolism by miR-1224-5p/FOXM1 axis in colorectal cancer. Cancer Cell Int. 2021 Nov 6;21(1):596.
Zannetti A, Ilardi G, Link W. Tumor Microenvironment: Molecular Mechanisms and Signaling Pathways Involved in Metastatic Progression. Frontiers Media SA; 2021. 566 p.