Time kill kinetic analysis of Acorus calamus leaves mediated selenium nanoparticles against Streptococcus mutans and Lactobacillus sp
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Abstract
INTRODUCTION
Dental caries, commonly known as tooth decay, is a prevalent oral health problem caused by the colonization and proliferation of cariogenic bacteria in the oral cavity. Streptococcus mutans and certain Lactobacillus species are recognized as the primary etiological agents responsible for dental caries initiation and progression. Traditional antimicrobial agents used for oral hygiene maintenance and caries prevention have encountered challenges, including the development of bacterial resistance and adverse side effects. Nanotechnology has emerged as a promising approach in dentistry, offering novel strategies for combating dental caries and enhancing oral health. Selenium nanoparticles have gained considerable attention due to their unique physicochemical properties and broad-spectrum antimicrobial activity.
AIM AND OBJECTIVE
To analyze the Time kill kinetic analysis of Acorus calamus leaves-mediated selenium nanoparticles against Streptococcus mutans and Lactobacillus sp
Assessing the antimicrobial activity of Acorus calamus leaf-mediated selenium nanoparticles against Streptococcus mutans and Lactobacillus sp.
Determining the kinetics of microbial growth inhibition by measuring the changes in microbial populations over time.
Evaluating the concentration-dependent effects of Acorus calamus leaf-mediated selenium nanoparticles on the targeted microorganisms.
MATERIALS AND METHODS
Obtain fresh Acorus calamus leaves and wash them thoroughly to remove any dirt or debris. Chop the leaves into small pieces and grind them in a blender or mortar and pestle.Add a suitable solvent, such as ethanol or methanol, to the ground leaves and mix well. Allow the mixture to stand for a specific period (e.g., 24 hours) at room temperature to facilitate extraction.Filter the mixture using a filter paper or a fine mesh to obtain the Acorus calamus leaf extract. Store the extract in a sterile container for further use.
CONCLUSION
Time-kill kinetic analysis revealed that the Acorus calamus leaf-mediated selenium nanoparticles exhibited significant antimicrobial activity against both Streptococcus mutans and Lactobacillus species. The results demonstrated a dose-dependent reduction in bacterial viability over time, highlighting the nanoparticles' ability to inhibit the growth and proliferation of these cariogenic bacteria.
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References
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