Association between Insulin like Growth Factor-1 Gene Polymorphisms (Rs35767) And Progression of Rheumatoid Arthritis in Iraqi Patients

Abstract

Rheumatoid arthritis (RA) is a prevalent, chronic autoimmune condition that affects the synovial membranes and articular tissues of many joints. Aim: detect the association between rs35767 in the promoter of the IGF-1 gene with serum level of IGF-1 protein and the development of RA. The study included 120 RA patients, which are divided into two groups (newly diagnosed and treated) in addition to the third group consisted of 60 healthy individuals. Using sandwich ELISA technique to estimate serum level of IGF-1, IGF-1R, and IGFBP3 . designing specific primer to detect the rs35767 by tetra-arm technique. GG represented the dominant genotype in patients (47.5%) , 35% ,17.55 for AG and AA respectively .Results revealed that serum level of IGF-1,IGF-1R and IGFBP3 were present at higher levels in newly diagnosed patients than in patients receiving treatment, both groups being higher by a clear and significant amount when compared to healthy subjects .Conclusion rs35677 found to be more common in patients , and can play an important role in the genetic vulnerability to RA diseases