Antidiarrheal And Acute Toxicity Study Of Ethanolic Extract Of Pyrus Pashia In Swiss Albino Mice

Main Article Content

Damit Kumar
Dr. Israr Ali


Diarrheal remains a major health concern in underdeveloped nations, causing millions of deaths and billions of episodes annually, according to the World Health Organization. This study investigated the antidiarrheal potential of Pyrus Pashiain ethanolic fruit extract using Wistar albino mice, with concurrent assessment of acute toxicity. The ethanolic extract exhibited significant antidiarrheal activity, reducing the defecation rate by up to 78.33% at 600 mg/kg, comparable to loperamide (100%). The mechanism involves denaturing proteins and forming protein tannates, reducing intestinal mucosa permeability. The LD50 for the ethanolic fruit extract was determined to be 10,715 mg/kg, indicating its safety. This study validates the traditional use of Pyrus Pashiain ethanolic fruit extract as a herbal remedy for diarrheal, showcasing both its efficacy and safety, contributing to the exploration of natural treatments for this prevalent health issue

Article Details

How to Cite
Damit Kumar, & Dr. Israr Ali. (2023). Antidiarrheal And Acute Toxicity Study Of Ethanolic Extract Of Pyrus Pashia In Swiss Albino Mice. Journal for ReAttach Therapy and Developmental Diversities, 6(8s), 761–765.
Author Biographies

Damit Kumar

Research scholar, IEC School of Pharmacy, IEC University, Baddi (HP),

Dr. Israr Ali

Professor, IEC School of Pharmacy, IEC University, Baddi (HP), India


Paniagua GL, Monroy E, García-González O, Alonso J, Negrete E, Vaca S. Two or more enteropathogens are associated with diarrhoea in Mexican children. Ann Clin Microbiol Antimicrob. 2007; 28; 6:17.

Adeyemi OO, Okpo SO, Adesaya AA. Gastrointestinal activity of the aqueous extract of a Nigerian poly herbal preparation. West Afr J Pharmacol Drug Res.2003; 19 (1&2):22-27.

Adjanohoun EJ, Ahyi MRA, Aké AL, Alia AM, Amai CA, Gbile ZO, et al. Traditional medicine and pharmacopoeia - contribution to ethnobotanical and floristic studies in Uganda. Scientific, Technical and Research Commission of the Organization of the African Unity. 1993; 317-318.

Farthing MJG. Novel targets for the control of secretory diarrhea. Gut. 2002; 50(Suppl 3).

Woodson RF. Statistical Methods for the Analysis of Biomedical Data. Wiley Series in Probability and Mathematical Statistics. In: New York: Wiley; 1987;315–316

Awouters F, Niemegeers CJ, Lenaerts FM, Janssen PA. Delay of castor oil diarrhea in rats: a new way to evaluate inhibitors of prostaglandin biosynthesis. J Pharm Pharmacol. 1978;30(1):41-5.

Gnanasekar N, Perianayagam J B. Influence of sodium curcuminate on castor oil-induced diarrhea in rats. Indian J Pharmacol 2004; 36:177-8

Harborne JB. Methods of plant analysis. Phytochemical Methods: A Guide to modern techniques of plant analysis. 3rd ed. London, UK: Chapman and Hall. 1998; 1-30.

Miller LC, Tainter ML. Estimation of the LD50 and its error by means of logarithmic probit graph paper. Proc Soc Exp Biol Med. 1944; 57:261–264.

Ghosh MN. Fundamentals of Experimental Pharmaclogy. 4th ed. Calcutta, India: Scientific Book Agency Calcutta. 1996.

Al-Ali A, Alkhawajah A, Randhawa M.A, Shaikh NA. Oral and intraperitoneal LD 50 of thymoquine, an active principle of Nigella sativa, in mice and rats. J Med Coll Abbotabad. 2008; 2: 25-27