Detection Of Urea By Polyaniline Based Modified Sensor With Geranium Particles.
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Abstract
A novel amperometric urea biosensor has been developed for selective and quantitative recognition of urea by immobilizing urease onto polyaniline (PANI) based nanocomposite graphite paste with geranium (Ga) electrode and monitoring the amperometric response caused by the immobilized urease reaction system. Urease immobilization on electrode was investigated using aamperometric method, and factors affecting its immobilization such as concentration of urease, pH was discussed in detail. Organized materials were characterized by analytical techniques such as UV-Vis, XRD and FE-SEM analysis. The performance of the developed urea biosensor was evaluated for polyaniline and polypyrrole, obtained urea biosensor exhibited shorter response time (3 s), wider linear range, lower detection limitand good stability with about 95% of the original response signal retained after 2 month for PANI
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