Effectiveness Of Ozonated Black Seed Oil On Full-Thickness Skin Defect Healing
Main Article Content
Abstract
Background: Inadequacies in wound treatment could lead to complications. Black seed oil (BSO) alone has antimicrobial and antioxidant properties. Ozone is known for its potential to improve the wound healing process through the reactive oxygen species (ROS) mechanism. However, the studies of ozonated black seed oil (OBSO) on wound healing are limited.
Objectives: To determine the effects of OBSO in full-thickness skin defect on the dorsal of Sprague Dawley (SD) rats, specifically on wound closure rate, epithelization, number of fibroblasts, and collagen distribution.
Methods: Full-thickness skin defect (10 mm) was created on dorsal of 60 SD rats that were divided into six groups: gentamycin, normal saline, BSO, OBSO at varied doses of 1.4, 1.8, and 2.2 g/dl (n=10). Half of the population in each group were terminated after three days of treatment, while the remain subjects were terminated after seven days. The wound area was measured with ImageJ computerized application and the skin tissue was excised for histopathological examination with HE and Van Giesson staining.
Results: The statistical analysis showed no significant differences in the wound area, reepithelization, and distribution of collagen after 3 and 7 days of treatment (p > 0.05), while there was a significant difference (p < 0.05) for the number of fibroblasts in the 3 and 7 days of treatment.
Conclusion: The application of OBSO gave no significant difference in enhancing wound healing compared to the control group after 3 and 7 days of treatment.
Article Details
References
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