Cytotoxic effect of nutmeg oleoresins assisted zinc oxide nanoparticles using breast cancer cell line
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Abstract
Cancer is a leading cause of death globally. Though there are different treatments available for cancer, the side effects associated are sometimes worse than the disease itself. Hence there is a constant search for new drug candidates to treat the condition and reduce the side effects. Many plant mediated nanoparticles are studied extensively and proved for their anticancer activity.Nutmeg is known for its diuretic, an antidepressant and memory-enhancing properties. It also exhibits great anti-oxidative capacity due to its polyphenolic compounds which may be used for many other uses of this plant. The aim of the present study was to evaluate the cytotoxic activity of nutmeg oleoresin mediated Zinc oxide nanoparticles against breast cancer cell lines. In the present study, nutmeg oleoresin mediated ZnO nanoparticles were prepared initially and confirmed by measuring the absorbance between 350 nm to 700 nm using UV- Visible spectroscopy . It was further evaluated for its anticancer activity on breast cancer cell line . The Cells were treated with different concentrations (10, 20, 40, 60, 80, 100 μg/ml) for 24hrs. The zinc nanoparticles prepared from nutmeg oleoresin showed good cytotoxic activity. The percentage viability was reduced with increase in concentration of nutmeg oleoresin mediated ZnO nanoparticle.The 50% of inhibition was observed at a concentration of 40 μg/ml and was considered as the IC50 value . The present study concluded the dose dependent nutmeg oleoresin mediated zinc oxide nanoparticles on breast cancer cell line. Hence, nutmeg oleoresin mediated zinc nanoparticles may be recommended for its use as an anticancer drug after further invivo studies.
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References
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