Investigation of Astrovirus and IFN-γ Polymorphism in Iraqi Childhood Patients with Gastroenteritis

Main Article Content

Israa Habeeb Naser
Shakir H .Mohammed Al-Alwany

Abstract

Background: Human astroviruses are considered acute gastroenteritis agents (AGE) and are largely


reported in children worldwide. Astrovirus causes endemic childhood diarrhea; worldwide, it is responsible for 3-9% of diarrheal illness. Transmission is primarily person to person via the fecal-oral route and also via contaminated food and water. 


Objectives: To determine the rate of human Astrovirus infections genome and the role of IFN-γ in limitation the severity and prevalence of virus among children and infants with gastroenteritis by assess it Astrovirus genome by quantitative Real Time PCR and also IFN-γ by conventional polymerase chain reaction among study population.


Patients and methods: Case control  study was carried out on 150 children and infant aged between (6-120 months)  with gastroenteritis who attended the middle Euphrates hospitals for Maternity and Children as well as private laboratories, during the period from February 2021 till September 2019. Stool, blood, body fluids samples were collected from each participant and stored as frozen at -70 °C to RNA extraction Astrovirus genome and IFN-γ genome by using  qReal Time PCR and Conventional PCR test.


Results:  The mean of age in patients with gastroenteritis was (43.56) while the control group  (40.6) , also we found the infection in male more than female in percentage 58% for male and 42% for female. The  rate of human Astrovirus infection according to the quantitative real time PCR  was 18.1% (14 out of 77) while the negative result was 81.9 % (63 out of 77). The results of conventional  polymerase chain reaction for IFN-γ for detection the relation with the limitation of virus severity demonstrated that only 21.3 % (32 out of 150 ) was positive for IFN-γ while the negative result was 78.7 % (118 out of 150).


Conclusion: : The genome of the human Astrovirus genome appears to play a major role in gastroenteritis among infants and children, and IFN-γ has an important role in limitation the severity of gastroenteritis among infected individuals.

Article Details

How to Cite
Naser, I. H., & Al-Alwany, S. H. .Mohammed. (2022). Investigation of Astrovirus and IFN-γ Polymorphism in Iraqi Childhood Patients with Gastroenteritis. Journal for ReAttach Therapy and Developmental Diversities, 5(1), 104–113. Retrieved from https://jrtdd.com/index.php/journal/article/view/70
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References

Palombo, E. (2012). Astroviruses as causative agents of gastroenteritis. Microbiology Australia, 33(2), 77-79.‏

De Benedictis, P., Schultz-Cherry, S., Burnham, A., & Cattoli, G. (2011). Astrovirus infections in humans and animals–molecular biology, genetic diversity, and interspecies transmissions. Infection, Genetics and Evolution, 11(7), 1529-1544.‏

Vu, D. L., Bosch, A., Pintó, R. M., & Guix, S. (2017). Epidemiology of classic and novel human astrovirus: gastroenteritis and beyond. Viruses, 9(2), 33.‏

Zaki, M. E. S., Mashaly, G. E. S., Alsayed, M. A. L., & Nomir, M. M. (2020). Molecular study of human astrovirus in Egyptian children with acute gastroenteritis. Germs, 10(3), 167.‏

Mendenhall, I.H.; Smith, G.J.; Vijaykrishna, D. Ecological Drivers of Virus Evolution: Astrovirus as a Case Study. J. Virol. 2015, 89, 6978–6981. [CrossRef] [PubMed]

L. Magpili. (2020). Factors Promoting Online Distance Educational Programs for International Students and the Impacts of the Covid19 Pandemic. Journal of Online Engineering Education, 11(1), 09–20. Retrieved from http://onlineengineeringeducation.com/index.php/joee/article/view/38

Xiao, C.T.; Giménez-Lirola, L.G.; Gerber, P.F.; Jiang, Y.H.; Halbur, P.G.; Opriessnig, T. Identification and characterization of novel porcine astroviruses (PAstVs) with high prevalence and frequent co-infection of individual pigs with multiple PAstV types. J. Gen. Virol. 2013, 94, 570–582. [CrossRef] [PubMed]

Liu L, Oza S, Hogan D, Chu Y, Perin J, Zhu J, Lawn JE, Cousens S, Mathers C, Black RE. Global, regional, and national causes of under-5 mortality in 2000–15: an updated systematic analysis with implications for the sustainable development goals. Lancet. 2016;388(10063):3027–35. https:// doi. org/ 10. 1016/ S0140-6736(16) 31593-8.

Taco-Masias AA, Fernandez-Aristi AR, Cornejo-Tapia A, Aguilar-Luis MA, Del Valle LJ, Silva-Caso W, Zavaleta-Gavidia V, Weilg P, Cornejo-Pacherres H, Bazلn-Mayra J, Puyen ZM, Del Valle-Mendoza J. Gut microbiota in hospitalized children with acute infective gastroenteritis caused by virus or bacteria in a regional Peruvian hospital. PeerJ. 2020;8:e9964. https:// doi. org/ 10. 7717/ peerj. 9964.

Amaral MS, Estevam GK, Penatti M, Lafontaine R, Lima IC, Spada PK, Gabbay YB, Matos NB. The prevalence of norovirus, astrovirus and adenovirus infections among hospitalised children with acute gastroenteritis in Porto Velho, state of Rondônia, western Brazilian Amazon. Mem Inst Oswaldo Cruz. 2015;110(2):215–21. https:// doi. org/ 10. 1590/ 0074-02760 140381.

Huynen P, Mauroy A, Martin C, Savadogo LGB, Boreux R, Thiry E. Molecular epidemiology of norovirus infections in symptomatic and asymptomatic children from Bobo Dioulasso, Burkina Faso. J Clin Virol 2013;58:515–21.

Mitui TM, Bozdayi G, Ahmed S, Matsumoto T, Nishizono A, Kamruddin A. Detection and molecular characterization of diarrhoea causing viruses in single and mixed infections in children: a comparative study between Bangladesh and Turkey. J Med Virol 2014;86:1159–68.

Qazoui M, Oumzil H, Baassi L, El Omari N, Sadki K, Amzazi S. Rotavirus and norovirus infections among acute gastroenteritis children in Morocco. BMC Infect Dis 2014;14:1–9.

Tsague, B. N., Louya, V. M., Ntoumi, F., Adedoja, A., Vouvoungui, C. J., Peko, S. M., & Abena, A. A. (2020). Occurrence of human astrovirus associated with gastroenteritis among Congolese children in Brazzaville, Republic of Congo. International Journal of Infectious Diseases, 95, 142-147.‏

Baldridge MT, King KY, Boles NC, Weksberg DC, Goodell MA. Quiescent haematopoietic stem cells are activated by IFN-gamma in response to chronic infection. Nature (2010) 465:793–7. doi:10.1038/nature09135

Duque G, Huang DC, Dion N, Macoritto M, Rivas D, Li W, et al. Interferon- gamma plays a role in bone formation in vivo and rescues osteoporosis in ovariectomized mice. J Bone Miner Res (2011) 26:1472–83. doi:10.1002/ jbmr.350

Chen HM, Tanaka N, Mitani Y, Oda E, Nozawa H, Chen JZ, et al. Critical role for constitutive type I interferon signaling in the prevention of cellular transformation. Cancer Sci (2009) 100:449–56. doi:10.1111/j.1349-7006.2008. 01051.x

Castro, F., Cardoso, A. P., Gonçalves, R. M., Serre, K., & Oliveira, M. J. (2018). Interferon-gamma at the crossroads of tumor immune surveillance or evasion. Frontiers in immunology, 9, 847.‏

George, D., & Mallery, P. (2019). IBM SPSS statistics 26 step by step: A simple guide and reference. Routledge.‏

Taco-Masias AA, Fernandez-Aristi AR, Cornejo-Tapia A, Aguilar-Luis MA, Del Valle LJ, Silva-Caso W, Zavaleta-Gavidia V, Weilg P, Cornejo-Pacherres H, Bazلn-Mayra J, Puyen ZM, Del Valle-Mendoza J. Gut microbiota in hospitalized children with acute infective gastroenteritis caused by virus or bacteria in a regional Peruvian hospital. PeerJ. 2020;8:e9964. https:// doi. org/ 10. 7717/ peerj. 9964.

Lu L, Zhong H, Xu M, Su L, Cao L, Jia R, Xu J. Molecular and epidemiological characterization of human adenovirus and classic human astrovirus in children with acute diarrhea in Shanghai, 2017–2018. BMC Infect Dis. 2021;21(1):713. https:// doi. org/ 10. 1186/ s12879-021-06403-1.

Kumthip K, Khamrin P, Ushijima H, Maneekarn N. Molecular epidemiology of classic, MLB and VA astroviruses isolated from < 5 year-old children with gastroenteritis in Thailand, 2011–2016. Infect Genet Evol. 2018;65:373–9. https:// doi. org/ 10. 1016/j. meegid. 2018. 08. 024.

Lu L, Zhong H, Xu M, Su L, Cao L, Jia R, Xu J. Molecular and epidemiological characterization of human adenovirus and classic human astrovirus in children with acute diarrhea in Shanghai, 2017–2018. BMC Infect Dis. 2021;21(1):713. https:// doi. org/ 10. 1186/ s12879-021-06403-1.

Bergallo M, Galliano I, Daprà V, Rassu M, Montanari P, Tovo PA. Molecular detection of human astrovirus in children with gastroenteritis, Northern Italy. Pediatr Infect Dis J. 2018;37(8):738–42. https:// doi. org/ 10. 1097/ INF. 00000 00000 001899.

Varela MF, Rivadulla E, Lema A, Romalde JL. Human sapovirus among outpatients with acute gastroenteritis in Spain: a 1-year study. Viruses.2019;11(2):144. https:// doi. org/ 10. 3390/ v1102 0144

Luo, X., Deng, J. K., Mu, X. P., Yu, N., & Che, X. (2021). Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China. BMC gastroenterology, 21(1), 1-8.‏

Biscaro V, Piccinelli G, Gargiulo F, Ianiro G, Caruso A, Caccuri F, De Francesco MA. Detection and molecular characterization of enteric viruses in children with acute gastroenteritis in Northern Italy. Infect Genet Evol. 2018;60:35–41. https:// doi. org/ 10. 1016/j. meegid. 2018. 02. 011.

Lekana-Douki SE, Kombila-Koumavor C, Nkogue D, Drosten C, Drexler JF, Leroy EM. Molecular epidemiology of enteric viruses and genotyping of rotavirus A, adenovirus and astrovirus among children under 5 years old in Gabon. Int J Infect Dis 2015;34:90–5, doi:http://dx.doi.org/10.1016/j.ijid.2015.03.009.

Ayolabi C, Ojo D, Akpan I. Astrovirus infection in children in Lagos, Nigeria. Afr J Infect Dis 2012;6(1):1–4, doi:http://dx.doi.org/10.4314/ajid.v6i1.77733.

Ali Karimi. (2022). Effect of Probiotics (Direct-Fed Microbials) in Poultry Production: A Comprehensive Review. Revista Electronica De Veterinaria, 72 - 82. Retrieved from https://www.veterinaria.org/index.php/REDVET/article/view/142

Siqueira JAM, Darleise de Souza O, Nascimento de Carvalho TC, Portal TM, Aguiar JMC, Damascena da Silva L, et al. Astrovirus infection in hospitalized children: molecular, clinical andepidemiological features. J Clin Virol 2017;17:1386–6532.

Khumela, R., Kabue, J. P., Traore, A. N., & Potgieter, N. (2021). Human Astrovirus in Symptomatic and Asymptomatic Children: A Cross-Sectional Study on Hospitalized and Outpatients from Rural Communities of South Africa between 2017–2021. Pathogens, 10(11), 1398.‏

Guix S, Perez-Bosque A, Miro L, Moreto M, Bosch A, Pinto RM. 2015. Type I interferon response is delayed in human astrovirus infections. PLoS One 10:e0123087. http://dx.doi.org/10.1371/journal.pone.0123087.

Marvin, S. A., Huerta, C. T., Sharp, B., Freiden, P., Cline, T. D., & Schultz-Cherry, S. (2016). Type I interferon response limits astrovirus replication and protects against increased barrier permeability in vitro and in vivo. Journal of virology, 90(4), 1988-1996.

Carsetti, R., Quintarelli, C., Quinti, I., Mortari, E. P., Zumla, A., Ippolito, G., & Locatelli, F. (2020). The immune system of children: the key to understanding SARS-CoV-2 susceptibility?. The Lancet Child & Adolescent Health, 4(6), 414-416.‏

Cortez, V., Meliopoulos, V. A., Karlsson, E. A., Hargest, V., Johnson, C., & Schultz-Cherry, S. (2017). Astrovirus biology and pathogenesis. Annual review of virology, 4, 327-348.‏